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Using glucophage for fertility Another drug they sometimes use is glucophage , it makes you less insulin resistant. 282 CHARACTERIZATION OF ZEBRAFISH MUTANTS SHOWING ABNORMAL RPE DEVELOPMENT LEADING TO MALPOSITIONED AND PARTIALLY FUSED EYECUPS DAHM R, ROJAS-MUNOZ A, NUESSLEIN-VOLHARD C Max-Planck-Institute for Developmental Biology The zebrafish is a favourite model organism for biologists studying developmental processes. Their embryonic development is very rapid, eg. by day 3, the visual system of the zebrafish is functional. In order to identify genes involved in the development of the vertebrate eye, we have recently performed a large-scale mutagenesis screen, which identified 245 mutants. Amongst others, two mutants were chosen for further analysis due to their highly specific and unusual eye phenotype. At the histological level the eyes are fused at the midline and the retinal pigment epithelium RPE ; is strongly reduced at day 5. Time course analyses of mutants using both histology and mRNA in situ hybridization revealed that during early embryogenesis eye development is delayed and apoptosis is increased in the region of the presumptive RPE. Accordingly, markers of the presumptive and mature RPE are not detectable in mutant embryos. In contrast, markers of early and late neural retina NR ; development are expressed, albeit with slight variations from the wt pattern. This suggests that the developmental program leading to the NR is not affected by the reduction or absence of the RPE. Though the NR develops normally as assessed by histological sections, the eyecups sink into the brain and fuse at around day 5, probably due to the absence of the RPE. The lack of RPE also affects the expression of diencephalon markers, indicative of a cell fate change and or a boundary disruption between the brain and the eye. Whether the prospective RPE is improperly specified and dies or transdifferentiates into NR is as yet unclear. 283 CLINICAL AND MOLECULAR ANALYSIS OF ITALIAN FAMILIES WITH CHOROIDEREMIA and hyzaar. 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